Differential expression analysis

R ile limma kullanarak Diferansiyel Ekspresyon Analizi

John Blischak

Instructor

What is the goal of a differential expression analysis?

Identify the genes that are associated with a phenotype of interest
Examples:
- The response to a stimulus like a drug
- Changes during development
- The effect of a genetic mutation

Why differential expression?

Novelty
- Are there additional genes of interest?
Context
- Is the measurement for a given gene unique or common?
Systems
- Which biological pathways are important?

Many steps to complete an experiment

Design study
Perform experiment
Collect data
Pre-process data
Explore data
Test data
Interpret results
Share results

Caveats

Measurements are relative, not absolute
Statistical methods cannot rescue a poorly designed study

Let's practice!

R ile limma kullanarak Diferansiyel Ekspresyon Analizi

Preparing Video For Download...