Introduction to RNA-Seq

RNA-Seq with Bioconductor in R

Mary Piper

Bioinformatics Consultant and Trainer

genome

RNA-Seq with Bioconductor in R

nucleotides

RNA-Seq with Bioconductor in R

DNA to RNA

RNA-Seq with Bioconductor in R

gene structure

RNA-Seq with Bioconductor in R

gene expression

RNA-Seq with Bioconductor in R

sickle cells

RNA-Seq with Bioconductor in R

RNA-Seq questions

  • What genes are differentially expressed between sample groups?

  • Are there any trends in gene expression over time or across conditions.

  • Which groups of genes change similarly over time or across conditions.

  • What processes or pathways are important for my condition of interest?

RNA-Seq with Bioconductor in R

Let's practice!

RNA-Seq with Bioconductor in R

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