Count normalization
RNA-Seq with Bioconductor in R
Mary Piper
Bioinformatics Consultant and Trainer
Count normalization
Library depth normalization
Library composition effect
DESeq2 normalization
Normalized counts: calculation
dds_wt <- estimateSizeFactors(dds_wt)sizeFactors(dds_wt)
Normalized counts: extraction
normalized_wt_counts <- counts(dds_wt, normalized=TRUE)View(normalized_wt_counts)
