Differential expression analysis
RNA-Seq with Bioconductor in R
Mary Piper
Bioinformatics Consultant and Trainer
Differential expression analysis: DESeq2 vignette
vignette(DESeq2)
Bringing in data for DESeq2
# Read in raw counts
wt_rawcounts <- read.csv("fibrosis_wt_rawcounts.csv")
View(wt_rawcounts)
Bringing in data for DESeq2: metadata
# Read in metadata
wt_metadata <- read.csv("fibrosis_wt_metadata_unordered.csv")
View(wt_metadata)
